Plant endogenous gene tRNALeu nucleic acid detection kit: 1-Hour Rapid On-Site qPCR Screening
Plant endogenous gene tRNALeu nucleic acid detection kit by DHelix is a highly sensitive real-time PCR (qPCR)-based kit designed for quality control and internal verification of plant-derived DNA in agricultural supply chains.
1-Hour Rapid Result: Get 1-hour on-site qPCR data—100% compatible with mainstream PCR systems from Bio-Rad & Thermo Fisher.
Maximum Sensitivity: High-efficiency verification of plant-derived DNA to actively eliminate false-negative diagnostic results in high-throughput screening.
Zero Cross-Reactivity: 100% elimination of false positives in complex agricultural matrices (raw grains, corn meal, soybean meal, and mixed feeds).
Industrial Internal Control: Perfectly designed to skip tedious official lab delays for private factory self-screening.
Advanced qPCR Technology for GMO Screening and Identity Preservation:
TaqMan® Probe-based Quality Control Strategy:The system utilizes a probe-based real-time PCR approach specifically optimized for verifying plant endogenous reference genes. Compared to dye-based systems, it provides exceptional tolerance to complex agricultural sample matrices, ensuring precise amplification of template DNA even in the presence of heavy background organic extraction residues.Internal Verification & False-Negative Prevention:This assay serves as a critical quality control gatekeeper in high-throughput screening pipelines. By simultaneously or independently tracking the highly conserved plant endogenous gene sequence, the system monitors the entire workflow from nucleic acid extraction to enzymatic amplification, successfully preventing costly false-negative diagnostic reports.High Specificity and Broad Dynamic Range:The assay targets universal plant genetic sequences with remarkable accuracy, demonstrating excellent linearity and amplification efficiency across a broad dynamic range. This guarantees a dependable, consistent validation benchmark across diverse botanical varieties and processed feed ingredients.Flexible Quality Assessment Methods:The system provides robust qualitative data to confirm the presence of viable amplifiable DNA, supporting private laboratory managers and grain sorting operators in validating sample integrity before pursuing downstream targeted GMO trait identification.Robust Performance and Reproducibility:Optimized master mixes, specialized primers, and advanced TaqMan probes ensure high stability and precise reproducibility across different testing batches, various thermocycler systems, and multi-location lab setups.User-friendly Quality Assurance Workflow:The ready-to-use reagent format drastically simplifies the laboratory quality control pipeline. It reduces operational hands-on time, eliminates standard mixing errors, and is perfectly engineered for both routine central lab audits and rapid mobile on-site field screening.
Advanced qPCR Technology for GMO Screening and Identity Preservation:
TaqMan® Probe-based Quality Control Strategy:The system utilizes a probe-based real-time PCR approach specifically optimized for verifying plant endogenous reference genes. Compared to dye-based systems, it provides exceptional tolerance to complex agricultural sample matrices, ensuring precise amplification of template DNA even in the presence of heavy background organic extraction residues.Internal Verification & False-Negative Prevention:This assay serves as a critical quality control gatekeeper in high-throughput screening pipelines. By simultaneously or independently tracking the highly conserved plant endogenous gene sequence, the system monitors the entire workflow from nucleic acid extraction to enzymatic amplification, successfully preventing costly false-negative diagnostic reports.High Specificity and Broad Dynamic Range:The assay targets universal plant genetic sequences with remarkable accuracy, demonstrating excellent linearity and amplification efficiency across a broad dynamic range. This guarantees a dependable, consistent validation benchmark across diverse botanical varieties and processed feed ingredients.Flexible Quality Assessment Methods:The system provides robust qualitative data to confirm the presence of viable amplifiable DNA, supporting private laboratory managers and grain sorting operators in validating sample integrity before pursuing downstream targeted GMO trait identification.Robust Performance and Reproducibility:Optimized master mixes, specialized primers, and advanced TaqMan probes ensure high stability and precise reproducibility across different testing batches, various thermocycler systems, and multi-location lab setups.User-friendly Quality Assurance Workflow:The ready-to-use reagent format drastically simplifies the laboratory quality control pipeline. It reduces operational hands-on time, eliminates standard mixing errors, and is perfectly engineered for both routine central lab audits and rapid mobile on-site field screening.
Why Private Enterprises Trust DHelix Molecular QC Series:
Rapid Results
Accelerated workflow provides accurate testing data in under 1 hours.
Exceptional Specificity
TaqMan® probe-based strategy ensures zero cross-reactivity.
High Sensitivity
Detects low copy numbers of plant endogenous reference genes for definitive sample validation.
User-friendly
Simplified operation requiring minimal hands-on time and training.
Standardized Workflow
Ready-to-use reagents ensure consistency across batches and labs.
GOLD Standard
Advanced TaqMan® technology for maximum diagnostic confidence.
Technical Features & System Highlights:
The system utilizes a probe-based real-time PCR approach, enabling higher specificity and improved tolerance to complex agricultural sample matrices compared to dye-based systems. It allows accurate detection even in samples with low target copy numbers or high background organic interference.
This system supports simultaneous detection of multiple targets within a single reaction (e.g., target GMO transgenic element + plant internal control). The built-in internal control effectively monitors nucleic acid extraction and amplification efficiency, minimizing the risk of false-negative results.
The assay is capable of detecting low copy numbers of target plant nucleic acids with excellent linearity across a wide dynamic range, ensuring reliable performance in both trace contamination screening and high-load verification conditions.
The system supports both absolute quantification (copy number per reaction or per unit volume) and relative quantification strategies, allowing users to evaluate presence and concentration levels under different application scenarios, from raw grains to mixed feeds.
Optimized primers, probes, and reaction conditions ensure high amplification efficiency and consistent results across different batches, instruments, and field operators.
The ready-to-use reagent format simplifies operation procedures, reduces hands-on time, and minimizes contamination risk, making it suitable for routine laboratory testing as well as on-site mobile field laboratories.
Technical Features & System Highlights （(Click to Expand):
The system utilizes a probe-based real-time PCR approach, enabling higher specificity and improved tolerance to complex agricultural sample matrices compared to dye-based systems. It allows accurate detection even in samples with low target copy numbers or high background organic interference.
This system supports simultaneous detection of multiple targets within a single reaction (e.g., target GMO transgenic element + plant internal control). The built-in internal control effectively monitors nucleic acid extraction and amplification efficiency, minimizing the risk of false-negative results.
The assay is capable of detecting low copy numbers of target plant nucleic acids with excellent linearity across a wide dynamic range, ensuring reliable performance in both trace contamination screening and high-load verification conditions.
The system supports both absolute quantification (copy number per reaction or per unit volume) and relative quantification strategies, allowing users to evaluate presence and concentration levels under different application scenarios, from raw grains to mixed feeds.
Optimized primers, probes, and reaction conditions ensure high amplification efficiency and consistent results across different batches, instruments, and field operators.
The ready-to-use reagent format simplifies operation procedures, reduces hands-on time, and minimizes contamination risk, making it suitable for routine laboratory testing as well as on-site mobile field laboratories.
Component Authentication:
The validation of sample DNA extraction quality and amplification efficiency is the absolute foundation of reliable molecular diagnostics, particularly in commercial agricultural supply chains and feed internal control. In high-throughput GMO screening, complex matrices like heavily processed soybean meal, DDGS, or mixed commercial feeds often contain PCR inhibitors that can lead to catastrophic false-negative results. DHelix’s plant endogenous gene tRNA-Leu nucleic acid detection kit serves as the definitive gold-standard internal control. By targeting the highly conserved, multi-copy tRNA-Leu (Transfer RNA Leucine) genetic sequence universally present across major economic plant species, this assay verifies the integrity of the extracted template DNA and the reaction chemistry itself. Delivering actionable verification data alongside your target pCaMV35S or tNOS screening within 45 minutes, this kit empowers private quality inspectors and grain export authorities to eliminate diagnostic blind spots, prevent compliance failures, and execute flawless bio-security internal controls.
Component Authentication (Click to Expand):
The validation of sample DNA extraction quality and amplification efficiency is the absolute foundation of reliable molecular diagnostics, particularly in commercial agricultural supply chains and feed internal control. In high-throughput GMO screening, complex matrices like heavily processed soybean meal, DDGS, or mixed commercial feeds often contain PCR inhibitors that can lead to catastrophic false-negative results. DHelix’s plant endogenous gene tRNA-Leu nucleic acid detection kit serves as the definitive gold-standard internal control. By targeting the highly conserved, multi-copy tRNA-Leu (Transfer RNA Leucine) genetic sequence universally present across major economic plant species, this assay verifies the integrity of the extracted template DNA and the reaction chemistry itself. Delivering actionable verification data alongside your target pCaMV35S or tNOS screening within 45 minutes, this kit empowers private quality inspectors and grain export authorities to eliminate diagnostic blind spots, prevent compliance failures, and execute flawless bio-security internal controls.
Plant Endogenous tRNA-Leu Kit Specifications & QC Validation Workflow:
- Catalog No.：041122M
- Format： 48 T
- Description：This kit is used for the qualitative detection and quality control verification of plant endogenous tRNA-Leu genetic sequences in agricultural raw materials.
- Sample Types：Validated Agricultural & Processing Matrices: Raw Grains & Oilseeds (Corn kernels, whole soybeans, canola seeds); Bulk Feed Ingredients (Corn meal, soybean meal, DDGS, wheat bran, and plant-derived proteins); Processed Food & Feed Products (Mixed commercial feeds, corn starch, soy flour, and various complex plant-derived matrices).
- Protocol: 1. Sample Preparation & DNA Extraction; 2. Reagents Preparation; 3. Amplification Reaction (qPCR); 4. Data Analysis.
Handling Precautions & Storage(Click to Expand):
1. Storage Temperature: This reagent kit should be stored at -20°C. To prevent the reagents from deterioration, only take out the necessary amount of reagents from the freezer before use (In order to maintain the reagents’ performance, avoid unnecessary freezing and thawing).
2. Reagent Preparation: Thaw the reagents at room temperature. Before use, spin down the tubes to drop down the reagents staying on the tube wall or on the cap, and mix well the reagents and spin down again. Notice that fierce mixing should be avoided as it can inactivate the Taq DNA Polymerase.
3. Contamination Control: Positive Control contains a high number of copies. In order to prevent Positive Control from contaminating other samples or reagents, always spin down before opening the tube and open the cap of the tube as short as possible. Also add into the reaction tubes under the following order from blank control, sample solution (extracted DNA), and leave the adding of positive control to the last and make sure that all other tube caps are closed when adding it. Moreover, to avoid contamination, do not use Positive Control in any other way not written in this instruction (such as diluting the positive control or adding it to samples).
4. Segregation Policy: Keep positive control and suspect positive samples away from the reagents when handling.
5. Batch Integrity: If there is any reagent left, do not use it with other kits even if they are in the same lot.
6. Amplicon Management: The caps of the used reaction tubes should not be opened. Pay special attention not to accidentally open the cap when taking the tubes out of the instrument. Contamination of amplified products on other samples may not only cause false judgment of the test result but also pollute the testing area. In this case, a correct test result may not be obtained unless pollution is completely removed.
7. Disposal Protocol: Keep the cap of the used tube completely closed and after double bagging it with the sealable vinyl bag. To prevent the amplified products from dispersing, do not conduct autoclave sterilization treatment for disposal.
8. Tube Physical Maintenance: This kit should be stored at -20°C. Storing the kit at a temperature lower than -20°C or repeated freezing and thawing might cause cracks on the tubes.
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