DHelix GMO maize multiplex qPCR nucleic acid detection kit for MON810 Bt11 TC1507 GA21 events screening

GMO Maize Multiplex Detection Kit (MON810 / Bt11 / TC1507 / GA21): 1-Hour Rapid On-Site qPCR Screening

GMO Maize Multiplex Detection Kit (MON810 / Bt11 / TC1507 / GA21) by DHelix is a highly sensitive real-time PCR (qPCR)-based kit for rapid screening of the regulatory-sensitive major transgenic events in commercial maize and corn-derived feed ingredients.

  • ⚡ 1-Hour Rapid Result: Get 1-hour on-site qPCR data—100% compatible with DHelix PCR systems and other mainstream open-channel real-time PCR platforms.
  • 🔬 Maximum Sensitivity: Limit of detection down to 0.01% (or 10 copies) for tracing low-level event-specific contamination.
  • 🌐 Zero Cross-Reactivity: 100% elimination of false positives in complex agricultural matrices (raw grains, corn meal, soybean meal, and mixed feeds).
  • 🛡️ Industrial Internal Control: Perfectly designed to skip tedious official lab delays for private factory safety self-screening.
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Advanced qPCR Technology for GMO Screening and Identity Preservation:

TaqMan® Probe Multiplex Detection Strategy: The system utilizes a highly specialized, event-specific real-time PCR approach engineered for the simultaneous detection of major market-restricted GM maize traits, including MON810, Bt11, TC1507, and GA21. Compared to traditional single-target methods, this probe-based assay delivers exceptional tolerance to complex agricultural sample matrices, enabling precise amplification of target DNA even in heavily processed corn meal or multi-ingredient commercial feeds.
Junction-Targeted Design & Zero Cross-Reactivity: This system features advanced primers and TaqMan® probes specifically designed to target the unique flanking junction sequences of multiple integration sites. By targeting these precise genetic boundaries, the system ensures 100% elimination of false positives and guarantees zero cross-reactivity with other commercial genetically modified crops or native plant DNA.
High Sensitivity for Trace Contamination Screening: The assay is capable of detecting exceptionally low copy numbers of target transgenic sequences with stable linearity across a wide dynamic range. This high analytical sensitivity down to 0.01% ensures reliable quality control performance, allowing operators to confidently track trace-level adventitious presence during bulk grain reception.
Definitive Internal Control & Risk Mitigation: The system supports robust relative and absolute quantification strategies, helping quality control managers and private laboratory inspectors accurately evaluate GM contamination levels. This allows private enterprises to execute strict internal controls and quietly isolate unapproved corn cargo before customs or commercial audits.
Robust Performance Across Diverse Processing Matrices: Optimized reaction chemistries and high-fidelity polymerases ensure consistent amplification efficiency and reproducible diagnostic results across various corn-derived by-products, including whole corn kernels, corn starch, and distillers dried grains with solubles (DDGS).
User-Friendly Workflow for On-Site Field Screening: The ready-to-use reagent format drastically simplifies the laboratory verification pipeline. It eliminates traditional master mix preparation errors and minimizes hands-on time, making it perfectly suited for routine central lab compliance audits as well as rapid mobile field testing at port laboratories.
TaqMan® Probe Multiplex Detection Strategy: The system utilizes a highly specialized, event-specific real-time PCR approach engineered for the simultaneous detection of major market-restricted GM maize traits, including MON810, Bt11, TC1507, and GA21. Compared to traditional single-target methods, this probe-based assay delivers exceptional tolerance to complex agricultural sample matrices, enabling precise amplification of target DNA even in heavily processed corn meal or multi-ingredient commercial feeds.
Junction-Targeted Design & Zero Cross-Reactivity: This system features advanced primers and TaqMan® probes specifically designed to target the unique flanking junction sequences of multiple integration sites. By targeting these precise genetic boundaries, the system ensures 100% elimination of false positives and guarantees zero cross-reactivity with other commercial genetically modified crops or native plant DNA.
High Sensitivity for Trace Contamination Screening: The assay is capable of detecting exceptionally low copy numbers of target transgenic sequences with stable linearity across a wide dynamic range. This high analytical sensitivity down to 0.01% ensures reliable quality control performance, allowing operators to confidently track trace-level adventitious presence during bulk grain reception.
Definitive Internal Control & Risk Mitigation: The system supports robust relative and absolute quantification strategies, helping quality control managers and private laboratory inspectors accurately evaluate GM contamination levels. This allows private enterprises to execute strict internal controls and quietly isolate unapproved corn cargo before customs or commercial audits.
Robust Performance Across Diverse Processing Matrices: Optimized reaction chemistries and high-fidelity polymerases ensure consistent amplification efficiency and reproducible diagnostic results across various corn-derived by-products, including whole corn kernels, corn starch, and distillers dried grains with solubles (DDGS).
User-Friendly Workflow for On-Site Field Screening: The ready-to-use reagent format drastically simplifies the laboratory verification pipeline. It eliminates traditional master mix preparation errors and minimizes hands-on time, making it perfectly suited for routine central lab compliance audits as well as rapid mobile field testing at port laboratories.

Why Private Enterprises Trust DHelix GMO Detection Kit:

Rapid Results

Accelerated workflow provides accurate testing data in within 1 hours.

Exceptional Specificity

TaqMan® probe-based strategy ensures zero cross-reactivity.

High Sensitivity

Detects low copy numbers of target transgenic elements for early warning.

User-friendly

Simplified operation requiring minimal hands-on time and training.

Standardized Workflow

Ready-to-use reagents ensure consistency across batches and labs.

GOLD Standard

Advanced TaqMan® technology for maximum diagnostic confidence.

Technical Features & System Highlights:

TaqMan® Probe-based Detection Strategy:The system utilizes a probe-based real-time PCR approach, enabling higher specificity and improved tolerance to complex agricultural sample matrices compared to dye-based systems. It allows accurate detection even in samples with low target copy numbers or high background organic interference.Multiplex Detection Design:This system supports simultaneous detection of multiple targets within a single reaction (e.g., target GMO maize transgenic events + internal control). The built-in internal control effectively monitors nucleic acid extraction and amplification efficiency, minimizing the risk of false-negative results.High Sensitivity and Broad Dynamic Range:The assay is capable of detecting low copy numbers of target transgenic nucleic acids with excellent linearity across a wide dynamic range, ensuring reliable performance in both trace contamination screening and high-load verification conditions.Flexible Quantification Methods:The system supports both absolute quantification (copy number per reaction or per unit volume) and relative quantification strategies, allowing users to evaluate presence and concentration levels under different application scenarios, from raw grains to mixed feeds.Robust Performance and Reproducibility:Optimized primers, probes, and reaction conditions ensure high amplification efficiency and consistent results across different batches, instruments, and field operators.User-Friendly Workflow:The ready-to-use reagent format simplifies operation procedures, reduces hands-on time, and minimizes contamination risk, making it suitable for routine laboratory testing as well as on-site mobile field laboratories.
TaqMan® Probe-based Detection Strategy:The system utilizes a probe-based real-time PCR approach, enabling higher specificity and improved tolerance to complex agricultural sample matrices compared to dye-based systems. It allows accurate detection even in samples with low target copy numbers or high background organic interference.Multiplex Detection Design:This system supports simultaneous detection of multiple targets within a single reaction (e.g., target GMO maize transgenic events + internal control). The built-in internal control effectively monitors nucleic acid extraction and amplification efficiency, minimizing the risk of false-negative results.High Sensitivity and Broad Dynamic Range:The assay is capable of detecting low copy numbers of target transgenic nucleic acids with excellent linearity across a wide dynamic range, ensuring reliable performance in both trace contamination screening and high-load verification conditions.Flexible Quantification Methods:The system supports both absolute quantification (copy number per reaction or per unit volume) and relative quantification strategies, allowing users to evaluate presence and concentration levels under different application scenarios, from raw grains to mixed feeds.Robust Performance and Reproducibility:Optimized primers, probes, and reaction conditions ensure high amplification efficiency and consistent results across different batches, instruments, and field operators.User-Friendly Workflow:The ready-to-use reagent format simplifies operation procedures, reduces hands-on time, and minimizes contamination risk, making it suitable for routine laboratory testing as well as on-site mobile field laboratories.

Component Authentication:

The precise monitoring of event-specific transgenic traits is a critical safeguard in global agricultural commodity trading and livestock feed manufacturing. Major regulatory-sensitive GMO maize lines worldwide present significant operational and compliance risks if mixed into non-GMO streams. Because unauthorized or unapproved presence of genetic events can lead to severe border rejections, costly litigation, and catastrophic supply chain disruptions, relying on slow third-party laboratory verification introduces significant operational risks. DHelix’s GMO Maize Multiplex Detection Kit is engineered specifically for definitive, rapid on-site internal control across multiple restricted traits. Utilizing advanced TaqMan probe-based real-time PCR (qPCR) technology, this assay targets unique, highly specific junction sequences flanking the integration sites of key GM events, including MON810, Bt11, TC1507, and GA21, ensuring zero cross-reactivity with other commercial corn variants. Delivering accurate screening data in just 1 hour with a superior analytical sensitivity down to 0.01%, this kit empowers quality control managers, grain sorters, and private feed mill inspectors to detect trace low-level adventitious presence quietly and confidently long before official customs and regulatory clearance inspections.

The precise monitoring of event-specific transgenic traits is a critical safeguard in global agricultural commodity trading and livestock feed manufacturing. Major regulatory-sensitive GMO maize lines worldwide present significant operational and compliance risks if mixed into non-GMO streams. Because unauthorized or unapproved presence of genetic events can lead to severe border rejections, costly litigation, and catastrophic supply chain disruptions, relying on slow third-party laboratory verification introduces significant operational risks. DHelix’s GMO Maize Multiplex Detection Kit is engineered specifically for definitive, rapid on-site internal control across multiple restricted traits. Utilizing advanced TaqMan probe-based real-time PCR (qPCR) technology, this assay targets unique, highly specific junction sequences flanking the integration sites of key GM events, including MON810, Bt11, TC1507, and GA21, ensuring zero cross-reactivity with other commercial corn variants. Delivering accurate screening data in just 1 hour with a superior analytical sensitivity down to 0.01%, this kit empowers quality control managers, grain sorters, and private feed mill inspectors to detect trace low-level adventitious presence quietly and confidently long before official customs and regulatory clearance inspections.

GMO Maize Multiplex Detection Kit (MON810 / Bt11 / TC1507 / GA21)Specifications & GMO Screening Workflow:

  • Catalog No.：042082M/042152M
  • Format： 48 T
  • Description：This kit is used for the rapid screening and event-specific qualitative detection of major GMO maize traits (MON810, Bt11, TC1507, and GA21) in corn-derived components and agricultural raw materials.
  • Sample Types：Validated Corn & Processing Matrices: Raw Corn Grains (Whole corn kernels, crushed corn, corn screenings); Bulk Feed Ingredients (Corn meal, corn gluten meal, DDGS, corn bran); Processed Food & Feed Products (Mixed commercial feeds, corn starch, corn flour, and complex corn-derived matrices).
  • Protocol: 1. Sample Preparation & DNA Extraction; 2. Reagents Preparation; 3. Amplification Reaction (qPCR); 4. Data Analysis.

1. Storage Temperature: This reagent kit should be stored at -20°C. To prevent the reagents from deterioration, only take out the necessary amount of reagents from the freezer before use (In order to maintain the reagents’ performance, avoid unnecessary freezing and thawing).

2. Reagent Preparation: Thaw the reagents at room temperature. Before use, spin down the tubes to drop down the reagents staying on the tube wall or on the cap, and mix well the reagents and spin down again. Notice that fierce mixing should be avoided as it can inactivate the Taq DNA Polymerase.

3. Contamination Control: Positive Control contains a high number of copies. In order to prevent Positive Control from contaminating other samples or reagents, always spin down before opening the tube and open the cap of the tube as short as possible. Also add into the reaction tubes under the following order from blank control, sample solution (extracted DNA), and leave the adding of positive control to the last and make sure that all other tube caps are closed when adding it. Moreover, to avoid contamination, do not use Positive Control in any other way not written in this instruction (such as diluting the positive control or adding it to samples).

4. Segregation Policy: Keep positive control and suspect positive samples away from the reagents when handling.

5. Batch Integrity: If there is any reagent left, do not use it with other kits even if they are in the same lot.

6. Amplicon Management: The caps of the used reaction tubes should not be opened. Pay special attention not to accidentally open the cap when taking the tubes out of the instrument. Contamination of amplified products on other samples may not only cause false judgment of the test result but also pollute the testing area. In this case, a correct test result may not be obtained unless pollution is completely removed.

7. Disposal Protocol: Keep the cap of the used tube completely closed and after double bagging it with the sealable vinyl bag. To prevent the amplified products from dispersing, do not conduct autoclave sterilization treatment for disposal.

8. Tube Physical Maintenance: This kit should be stored at -20°C. Storing the kit at a temperature lower than -20°C or repeated freezing and thawing might cause cracks on the tubes.

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