Maize endogenous gene nucleic acid detection kit: 1-Hour Rapid On-Site qPCR Screening

Maize endogenous gene nucleic acid detection kit by DHelix is a highly sensitive real-time PCR (qPCR)-based kit designed for quality control and internal verification of maize-derived DNA in agricultural supply chains.

⚡ 1-Hour Rapid Result: Get 1-hour on-site qPCR data—100% compatible with DHelix PCR systems and other mainstream open-channel real-time PCR platforms. DHelix PCR systems
🔬 Maximum Sensitivity: High-efficiency verification of maize-specific internal reference gene to actively eliminate false-negative diagnostic results in high-throughput screening. maize-specific internal reference gene
🌐 Zero Cross-Reactivity: 100% elimination of false positives in complex agricultural matrices (raw grains, corn meal, soybean meal, and mixed feeds).
🛡️ Industrial Internal Control: Perfectly designed to skip tedious official lab delays for private factory safety self-screening.

Advanced qPCR Technology for GMO Screening and Identity Preservation:

The system utilizes a probe-based real-time PCR approach optimized specifically for verifying maize endogenous reference genes. Compared to dye-based systems, it provides exceptional tolerance to complex agricultural sample matrices, ensuring precise amplification of template DNA even in the presence of heavy background organic extraction residues. This assay serves as a critical quality control gatekeeper in high-throughput screening pipelines. By tracking the highly conserved maize endogenous gene sequence, the system monitors the entire workflow from nucleic acid extraction to enzymatic amplification, successfully preventing costly false-negative diagnostic results. The system features advanced primers and TaqMan® probes designed to target maize-specific genetic sequences with remarkable accuracy, demonstrating excellent linearity and amplification efficiency across a broad dynamic range. This guarantees a dependable, consistent validation benchmark across processed feed ingredients. Optimized master mixes, specialized primers, and advanced TaqMan probes ensure high stability and precise reproducibility across different testing batches and various real-time PCR platforms. The ready-to-use reagent format drastically simplifies the laboratory quality control pipeline, reduces operational hands-on time, eliminates standard mixing errors, and is perfectly engineered for both routine central lab audits and rapid mobile on-site field screening.

Advanced qPCR Technology for GMO Screening and Identity Preservation:

Why Private Enterprises Trust DHelix Molecular QC Series:

Rapid Results

Accelerated workflow provides accurate testing data in under 1 hours.

Exceptional Specificity

TaqMan® probe-based strategy ensures zero cross-reactivity with non-target DNA

High Sensitivity

Detects low copy numbers of plant endogenous reference genes for definitive sample validation.

User-friendly

Simplified operation requiring minimal hands-on time and training.

Standardized Workflow

Ready-to-use reagents ensure consistency across batches and labs.

GOLD Standard

Advanced TaqMan® technology for maximum diagnostic confidence.

Technical Features & System Highlights:

The system utilizes a probe-based real-time PCR approach, enabling higher specificity and improved tolerance to complex agricultural sample matrices compared to dye-based systems. It allows accurate detection even in samples with low target copy numbers or high background organic interference. This system serves as a dedicated quality control gatekeeper, utilizing a highly conserved maize-derived internal control to effectively monitor nucleic acid extraction and amplification efficiency, minimizing the risk of false-negative results. The assay is capable of detecting low copy numbers of maize-specific endogenous nucleic acids with excellent linearity across a wide dynamic range, ensuring reliable performance in both routine screening and high-load verification conditions. The system supports both absolute quantification (copy number per reaction or per unit volume) and relative quantification strategies, allowing users to evaluate presence and concentration levels under different application scenarios, from raw grains to mixed feeds. Optimized primers, probes, and reaction conditions ensure high amplification efficiency and consistent results across different batches, instruments, and field operators. The ready-to-use reagent format drastically simplifies the laboratory quality control pipeline, reduces operational hands-on time, eliminates standard mixing errors, and is perfectly engineered for both routine central lab compliance audits as well as rapid mobile on-site field screening.

Component Authentication:

The validation of sample DNA extraction quality and amplification efficiency is the absolute foundation of reliable molecular diagnostics, particularly in commercial agricultural supply chains and feed internal control. In high-throughput GMO screening, complex matrices like heavily processed corn meal, DDGS, or mixed commercial feeds often contain PCR inhibitors that can lead to catastrophic false-negative results. DHelix’s Maize Endogenous Gene Nucleic Acid Detection Kit serves as the definitive gold-standard internal control. By targeting the highly conserved, maize-specific endogenous reference gene sequence universally present across major economic corn varieties, this assay verifies the integrity of the extracted template DNA and the reaction chemistry itself. Delivering actionable verification data alongside your target screening within 1 hour, this kit empowers private quality inspectors and grain export authorities to eliminate diagnostic blind spots, prevent compliance failures, and execute flawless bio-security internal controls.

Component Authentication (Click to Expand):

Maize Endogenous Gene Kit Specifications & QC Validation Workflow:

Catalog No.：042202M
Format： 48 T
Description：This kit is used for the qualitative detection and quality control verification of maize endogenous reference gene genetic sequences in agricultural raw materials and feeds.
Sample Types：Validated Agricultural & Processing Matrices: Raw Grains (Corn kernels, whole corn, crushed corn); Bulk Feed Ingredients (Corn meal, corn gluten meal, DDGS, corn bran); Processed Food & Feed Products (Mixed commercial feeds, corn starch, corn flour, and various complex corn-derived matrices).
Protocol: 1. Sample Preparation & DNA Extraction; 2. Reagents Preparation; 3. Amplification Reaction (qPCR); 4. Data Analysis.

Handling Precautions & Storage(Click to Expand):

1. Storage Temperature: This reagent kit should be stored at -20°C. To prevent the reagents from deterioration, only take out the necessary amount of reagents from the freezer before use (In order to maintain the reagents’ performance, avoid unnecessary freezing and thawing).

2. Reagent Preparation: Thaw the reagents at room temperature. Before use, spin down the tubes to drop down the reagents staying on the tube wall or on the cap, and mix well the reagents and spin down again. Notice that fierce mixing should be avoided as it can inactivate the Taq DNA Polymerase.

3. Contamination Control: Positive Control contains a high number of copies. In order to prevent Positive Control from contaminating other samples or reagents, always spin down before opening the tube and open the cap of the tube as short as possible. Also add into the reaction tubes under the following order from blank control, sample solution (extracted DNA), and leave the adding of positive control to the last and make sure that all other tube caps are closed when adding it. Moreover, to avoid contamination, do not use Positive Control in any other way not written in this instruction (such as diluting the positive control or adding it to samples).

4. Segregation Policy: Keep positive control and suspect positive samples away from the reagents when handling.

5. Batch Integrity: If there is any reagent left, do not use it with other kits even if they are in the same lot.

6. Amplicon Management: The caps of the used reaction tubes should not be opened. Pay special attention not to accidentally open the cap when taking the tubes out of the instrument. Contamination of amplified products on other samples may not only cause false judgment of the test result but also pollute the testing area. In this case, a correct test result may not be obtained unless pollution is completely removed.

7. Disposal Protocol: Keep the cap of the used tube completely closed and after double bagging it with the sealable vinyl bag. To prevent the amplified products from dispersing, do not conduct autoclave sterilization treatment for disposal.

8. Tube Physical Maintenance: This kit should be stored at -20°C. Storing the kit at a temperature lower than -20°C or repeated freezing and thawing might cause cracks on the tubes.

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