DHelix GMO soybean multiplex qPCR nucleic acid detection kit for GTS40-3-2 MON89788 DAS44406 events screening

GMO Soybean Multiplex Detection Kit (GTS40-3-2 / MON89788 / DAS44406): 1-Hour Rapid On-Site qPCR Screening

GMO Soybean Multiplex Detection Kit (GTS40-3-2 / MON89788 / DAS44406) by DHelix is a highly sensitive real-time PCR (qPCR)-based kit for rapid screening of the regulatory-sensitive major transgenic events in commercial soybean and soy-derived feed ingredients.

⚡ 1-Hour Rapid Result: Get 1-hour on-site qPCR data—100% compatible with DHelix PCR systems and other mainstream open-channel real-time PCR platforms.
🔬 Maximum Sensitivity: Limit of detection down to 0.01% (or 10 copies) for tracing low-level soybean event-specific contamination.
🌐 Zero Cross-Reactivity: 100% elimination of false positives in complex agricultural matrices (raw soybean, soy meal, soy flakes, and mixed feeds).
🛡️ Industrial Internal Control: Perfectly designed to skip tedious official lab delays for private factory safety self-screening.
 
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Advanced qPCR Technology for GMO Screening and Identity Preservation:

The system utilizes a probe-based multiplex qPCR approach engineered for the simultaneous detection of major regulatory-sensitive GM soybean traits, including GTS40-3-2, MON89788, and DAS44406. Compared to traditional single-target methods, this assay delivers exceptional tolerance to complex agricultural sample matrices, enabling precise amplification of target DNA even in heavily processed soy meal or multi-ingredient commercial feeds. Advanced primers and TaqMan® probes ensure 100% elimination of false positives and guarantee zero cross-reactivity with native plant DNA. This high analytical sensitivity down to 0.01% LOD ensures reliable quality control performance, allowing operators to track trace-level adventitious presence confidently during bulk grain reception. It serves as an essential tool for private enterprise internal control, helping quality managers quietly isolate unapproved soybean cargo before official customs and commercial audits. The ready-to-use reagent format drastically simplifies the laboratory quality control pipeline, reduces operational hands-on time, and is perfectly engineered for both routine central lab audits and rapid mobile on-site field screening within 1 hour.
The system utilizes a probe-based multiplex qPCR approach engineered for the simultaneous detection of major regulatory-sensitive GM soybean traits, including GTS40-3-2, MON89788, and DAS44406. Compared to traditional single-target methods, this assay delivers exceptional tolerance to complex agricultural sample matrices, enabling precise amplification of target DNA even in heavily processed soy meal or multi-ingredient commercial feeds. Advanced primers and TaqMan® probes ensure 100% elimination of false positives and guarantee zero cross-reactivity with native plant DNA. This high analytical sensitivity down to 0.01% LOD ensures reliable quality control performance, allowing operators to track trace-level adventitious presence confidently during bulk grain reception. It serves as an essential tool for private enterprise internal control, helping quality managers quietly isolate unapproved soybean cargo before official customs and commercial audits. The ready-to-use reagent format drastically simplifies the laboratory quality control pipeline, reduces operational hands-on time, and is perfectly engineered for both routine central lab audits and rapid mobile on-site field screening within 1 hour.

Why Private Enterprises Trust DHelix GMO Detection Kit:

Rapid Results

Accelerated workflow provides accurate testing data in within 1 hours.

Exceptional Specificity

TaqMan® probe-based strategy ensures zero cross-reactivity.

High Sensitivity

Detects low copy numbers of target transgenic elements for early warning.

User-friendly

Simplified operation requiring minimal hands-on time and training.

Standardized Workflow

Ready-to-use reagents ensure consistency across batches and labs.

GOLD Standard

Advanced TaqMan® technology for maximum diagnostic confidence.

Technical Features & System Highlights:

The system utilizes a probe-based real-time PCR approach, enabling higher specificity and improved tolerance to complex agricultural sample matrices compared to dye-based systems. It allows accurate detection even in samples with low target copy numbers or high background organic interference. This system supports simultaneous detection of multiple targets within a single reaction (e.g., target GMO soybean transgenic events + internal control). The built-in internal control effectively monitors nucleic acid extraction and amplification efficiency, minimizing the risk of false-negative results. The assay is capable of detecting low copy numbers of soybean-specific transgenic nucleic acids with excellent linearity across a wide dynamic range, ensuring reliable performance in both trace contamination screening and high-load verification conditions. The system supports both absolute quantification (copy number per reaction or per unit volume) and relative quantification strategies, allowing users to evaluate presence and concentration levels under different application scenarios, from raw soy grains to soy meal and mixed feeds. Optimized primers, probes, and reaction conditions ensure high amplification efficiency and consistent results across different batches, instruments, and field operators. The ready-to-use reagent format drastically simplifies the laboratory quality control pipeline, reduces operational hands-on time, eliminates standard mixing errors, and is perfectly engineered for both routine central lab compliance audits as well as rapid mobile on-site field screening.
 
The system utilizes a probe-based real-time PCR approach, enabling higher specificity and improved tolerance to complex agricultural sample matrices compared to dye-based systems. It allows accurate detection even in samples with low target copy numbers or high background organic interference. This system supports simultaneous detection of multiple targets within a single reaction (e.g., target GMO soybean transgenic events + internal control). The built-in internal control effectively monitors nucleic acid extraction and amplification efficiency, minimizing the risk of false-negative results. The assay is capable of detecting low copy numbers of soybean-specific transgenic nucleic acids with excellent linearity across a wide dynamic range, ensuring reliable performance in both trace contamination screening and high-load verification conditions. The system supports both absolute quantification (copy number per reaction or per unit volume) and relative quantification strategies, allowing users to evaluate presence and concentration levels under different application scenarios, from raw soy grains to soy meal and mixed feeds. Optimized primers, probes, and reaction conditions ensure high amplification efficiency and consistent results across different batches, instruments, and field operators. The ready-to-use reagent format drastically simplifies the laboratory quality control pipeline, reduces operational hands-on time, eliminates standard mixing errors, and is perfectly engineered for both routine central lab compliance audits as well as rapid mobile on-site field screening.
 

Component Authentication:

The precise monitoring of event-specific transgenic traits is a critical safeguard in global soybean trading and livestock feed manufacturing. Relying on slow third-party laboratory verification introduces significant operational risks for private enterprises. DHelix’s GMO Soybean Multiplex Detection Kit is engineered specifically for definitive, rapid on-site internal control across multiple restricted traits. Utilizing advanced TaqMan probe-based real-time PCR (qPCR) technology, this assay targets unique, highly specific junction sequences flanking the integration sites of key GM events, including GTS40-3-2, MON89788, and DAS44406, ensuring zero cross-reactivity with native variants. Delivering accurate screening data in just 1 hour with a 0.01% sensitivity, this kit empowers quality control managers and private feed mill inspectors to detect trace low-level adventitious presence quietly and confidently long before official customs and regulatory clearance inspections.
 
The precise monitoring of event-specific transgenic traits is a critical safeguard in global soybean trading and livestock feed manufacturing. Relying on slow third-party laboratory verification introduces significant operational risks for private enterprises. DHelix’s GMO Soybean Multiplex Detection Kit is engineered specifically for definitive, rapid on-site internal control across multiple restricted traits. Utilizing advanced TaqMan probe-based real-time PCR (qPCR) technology, this assay targets unique, highly specific junction sequences flanking the integration sites of key GM events, including GTS40-3-2, MON89788, and DAS44406, ensuring zero cross-reactivity with native variants. Delivering accurate screening data in just 1 hour with a 0.01% sensitivity, this kit empowers quality control managers and private feed mill inspectors to detect trace low-level adventitious presence quietly and confidently long before official customs and regulatory clearance inspections.
 

GMO Soybean Multiplex Detection Kit (GTS40-3-2 / MON89788/DAS44406)Specifications & GMO Screening Workflow:

  • Catalog No.：043092M
  • Format： 48 T
  • Description：This kit is used for the rapid screening and event-specific qualitative detection of major GMO soybean traits (GTS40-3-2, MON89788, and DAS44406) in soy-derived components and agricultural raw materials.
  • Sample Types：Validated Soybean & Processing Matrices: Raw Soy Grains (Soybeans, whole soy, crushed soy, soy screenings); Bulk Feed Ingredients (Soy meal, soy flakes, soy flour, soy hulls); Processed Food & Feed Products (Mixed commercial feeds, textured soy protein, and various complex soy-derived matrices).
  • Protocol: 1. Sample Preparation & DNA Extraction; 2. Reagents Preparation; 3. Amplification Reaction (qPCR); 4. Data Analysis.

1. Storage Temperature: This reagent kit should be stored at -20°C. To prevent the reagents from deterioration, only take out the necessary amount of reagents from the freezer before use (In order to maintain the reagents’ performance, avoid unnecessary freezing and thawing).

2. Reagent Preparation: Thaw the reagents at room temperature. Before use, spin down the tubes to drop down the reagents staying on the tube wall or on the cap, and mix well the reagents and spin down again. Notice that fierce mixing should be avoided as it can inactivate the Taq DNA Polymerase.

3. Contamination Control: Positive Control contains a high number of copies. In order to prevent Positive Control from contaminating other samples or reagents, always spin down before opening the tube and open the cap of the tube as short as possible. Also add into the reaction tubes under the following order from blank control, sample solution (extracted DNA), and leave the adding of positive control to the last and make sure that all other tube caps are closed when adding it. Moreover, to avoid contamination, do not use Positive Control in any other way not written in this instruction (such as diluting the positive control or adding it to samples).

4. Segregation Policy: Keep positive control and suspect positive samples away from the reagents when handling.

5. Batch Integrity: If there is any reagent left, do not use it with other kits even if they are in the same lot.

6. Amplicon Management: The caps of the used reaction tubes should not be opened. Pay special attention not to accidentally open the cap when taking the tubes out of the instrument. Contamination of amplified products on other samples may not only cause false judgment of the test result but also pollute the testing area. In this case, a correct test result may not be obtained unless pollution is completely removed.

7. Disposal Protocol: Keep the cap of the used tube completely closed and after double bagging it with the sealable vinyl bag. To prevent the amplified products from dispersing, do not conduct autoclave sterilization treatment for disposal.

8. Tube Physical Maintenance: This kit should be stored at -20°C. Storing the kit at a temperature lower than -20°C or repeated freezing and thawing might cause cracks on the tubes.

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